P-136: High Concentrations of Noscapine Induces Apoptosis in Stromal Cells of Endometriosis In Vitro

Background: Endometriosis is a benign hormone-dependent disease in women of reproductive age. There is no cure for eradicating the disease and there is need for new drugs. The aim of this study was to investigate the in vitro effect of noscapine as inducer of cell death on endometriotic stromal cells in patient&#039;s endometriosis in vitro. Materials and Methods: Endometrial biopsies from patient&#039;s endometriosis (n=8) were digested by enzymatic method (collagenase, 2 mg/ml). Stromal cells were collected by sequential filtration through nylon meshes (70 and 40 μm) and ficoll layering. The cells were divided to five groups: control and 10, 25, 50, and 100μM concentration of noscapine, and cultured for 48 hours. Cell viability was assessed by MTT assay; and cell morphology analyzed with Acridine orange (AO)-ethidium bromide (EB) double staining, and cell death was assessed by TUNEL assay. Data was analyzed by one way ANOVA. Results: Viability of endometrial stromal cells were 94.3, 88.4, 86.7, 86.3, 76.9% in control, 10, 25, 50 and 100 μM noscapine concentration in 48 hours respectively. Cell death increased in high concentrations of noscapine and TUNEL positive cells were increased in 10, 25, 50 and 100 μM noscapine concentration (7.48, 12.56, 14.44, 16.11% respectively) compared to control group (4.46%) in 48 hours (p<0.05). Conclusion: Noscapine increased apoptotic index in endometriotic stromal cells in dose and time dependent manner. It can suggest for endometriosis treatment.